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Evaluating skin‐protective materials against contact irritants and allergens
Author(s) -
Zhai Hongbo,
Willard Paul,
Maibach Howard I.
Publication year - 1998
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1998.tb05683.x
Subject(s) - wax , irritation , allergen , irritant contact dermatitis , chemistry , contact dermatitis , patch test , allergic contact dermatitis , lanolin , beeswax , diaper dermatitis , dermatology , alcohol , allergy , medicine , chromatography , organic chemistry , immunology
2 acute irritants and 1 allergen were selected: sodium lauryl sulfate (SLS) representative of irritant household and occupational contact dermatitis, the combination of ammonium hydroxide (NH 4 OH) and urea to simulate diaper dermatitis, and Rhus to evaluate the effect of model protective materials. The putative protective materials and vehicle were applied to both ventral forearms of 10 subjects in each group, according to a randomized code. Test materials were spread over a marked 2.0 cm 2 area, massaged in, allowed to dry for 30 min, and reapplied with another 30 min drying period. The model irritants and allergen were then applied (0.025 ml) to an Al‐test® occlusive patch, which in turn was placed for 24 h over each of the 8 designated sites. Inflammation was scored according to a clinical scale 72 h post‐application. Paraffin wax plus Acetulan® in cetyl alcohol, and beeswax plus Acetulan® in cetyl alcohol, markedly ( p < 0.001) suppressed SLS irritation. Paraffin wax plus beeswax in cetyl alcohol, and Acetulan® in cetyl alcohol reduced NH 4 OH and urea irritation ( p < 0.05), paraffin wax in cetyl alcohol significantly ( p < 0.01) decreasing Rhus allergic contact dermatitis. This model, provides an easy approach to screening protectants. Its clinical significance requires comparison with an open rather than an occluded challenge.