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Different pathways in irritant contact eczema?
Author(s) -
Grängsjö A.,
LeijonKuligowski A.,
To̊mä H.,
Roomans G. M.,
Lindberg M.
Publication year - 1996
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1996.tb02416.x
Subject(s) - irritant contact dermatitis , chemistry , epidermis (zoology) , irritation , contact dermatitis , tumor necrosis factor alpha , sodium , microbiology and biotechnology , immunology , biochemistry , allergy , biology , anatomy , organic chemistry
The epidermal response to 2 different irritants, nonanoic acid (NAA) and sodium lauryl sulfate (SLS), was investigated with 2 different methods. NAA 80% and SLS 4% were applied under occlusion for up to 24 h. Elemental changes were determined in cryosections by x‐ray microanalysis. Compared to unexposed skin a significantly higher sodium/potassium ratio was found after 6 h in NAA‐exposed skin and a lower ratio in SLS‐exposed. At 24 h both substances had induced similar changes, compatible with a cell injury. The findings demonstrate a time‐dependent NAA and SLS response, With reverse transcription potymerase chain reaction, the mRNA expression of interleukin‐1 alpha (IL‐1α;), ‐1beta (IL.‐1β), ‐6 (1L‐6), and ‐8 (IL‐8). tumor necrosis factor alpha (TNFα) and granulocyte macrophaye colony stimulating factor (GM‐CSF) in shave biopsies from irritated and unexposed skin was studied at 0. 4, 8 and 24 h NAA, but not SLS. induced an increase in mRNA expression for IL‐6. mRNA‐expression for GM‐CSF was increased after SLS exposure, but not after NAA. These findings indicate a time and substance dependent different in the up‐regulation of m‐RNA different cytokines in epidermis during the first 24 h of the irritant reaction. This might he the effect of differences in the irritants action on the cell membranes, which is also reflected by the differences found in the elemental content at 6 h.