Premium
Nickel‐sulphate‐induced contact dermatitis in the guinea pig maximization test: a dose‐response study
Author(s) -
Rohold Allan E.,
Nielsen Gitte D.,
Andersen Klaus E.
Publication year - 1991
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1991.tb01627.x
Subject(s) - sensitization , guinea pig , irritation , contact dermatitis , medicine , dose–response relationship , dermatology , chemistry , pharmacology , immunology , allergy
Nickel sulphate is a sensitizer in guinea pigs, but the frequency of sensitization varies from study to study. The dose‐response relationship for NiSO 4 6H 2 O was evaluated in the guinea pig maximization test in this study, 6 intradermal (0.01%–3.0% aq.) and 6 topical (0.25% 10.0% pet.) concentrations were chosen for induction and NiSO 4 6H 2 O 1% pet was used for challenge, based on the absence of skin irritation in a pilot study. Blind reading was performed. A logistic dose‐response model was applied to the challenge results. At 48 h, a linear relationship was obtained between the intradermal induction dose (but not topical dose) and the response, resulting in a maximum sensitization rate of 40% after 3% i.d. The reactivity disappeared at re‐challenge I week later. Following a booster closed patch on day 35, using NiSO 4 pet., the animals were challenged with NiSO 4 2% pet. and statistical analyses of 72‐h readings revealed a non‐linear dose‐response relationship, giving a maximum response Frequency of 40% after initial induction with NiSO 4 , 3% i.d. and 2% topical.