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Evaluation of a non‐invasive human and an in vitro cytotoxicity method as alternatives to the skin irritation test on rabbits
Author(s) -
Jacobs Guido A.,
Castellazzi Annamaria,
Dierickx Paul J.
Publication year - 1989
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1989.tb03202.x
Subject(s) - cytotoxicity , erythema , human skin , in vivo , irritation , in vitro , subclinical infection , chemistry , pharmacology , medicine , dermatology , pathology , immunology , biology , biochemistry , microbiology and biotechnology , genetics
5 substances were investigated for their primary irritant effect by means of different protocols. The cutaneous blood flow values (CBFV) were measured in humans: in a 1st series of experiments 12 h after application of the pure substances for 48 h and in a 2nd series of experiment 1, 24. 48 and 72 after application of the 10% diluted substances (subclinical concentration) for 3 h. Rabbit skin erythema scores were obtained 1, 24, 48 and 72 h after application of the undiluted substances for 4 h. The undine uptake inhibition assay on KB cells was included as a cytotoxicity test. Results obtained for both protocols on humans are compared with each other and with the test data obtained on rabbits, and with the cytotoxicity data. Application of the undiluted substances for 48 h on human skin or for only 3 h at 10% dilution resulted in the same ranking between the substances. The CBFV in the human correlated very well (r = 0.99) with erythema scores obtained on rabbits. A poor correlation was observed between the in vitro and the in vivo results. From this study, it can be concluded that CBFV measurement is a valid method For the detection of erythema at subclinical concentrations.