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Skin absorption from patch test systems
Author(s) -
Kim Hyung Ok,
Wester Ronald C.,
McMaster James A.,
Bucks Daniel A. W.,
Maibach Howard I.
Publication year - 1987
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1987.tb02701.x
Subject(s) - patch test , absorption (acoustics) , skin reaction , patch testing , dermatology , chemistry , contact dermatitis , medicine , materials science , allergy , immunology , composite material
The development of topical drug products requires testing for skin toxicology reactions. A variety of patch test systems are available with which chemicals are applied to skin. The purpose of this study was to determine the skin absorption of paraphenylenediamine (PPDA) from a variety of such systems. (14C)‐PPDA (1% pet., USP) was placed in a variety of patch lest systems at a concentration normalized to equal surface area (2 mg/mm 2 . Skin absorption was determined in the guinea pig by urinary excretion of 14 C. There was a six‐fold difference in the range of skin absorption ( p <0.02). In decreasing order, % skin absorption from the systems were Hill Top Chamber (53.4 ± 20.6)>Teflon Control Patch (29.8 ± 9.0)>Large Small Finn Chamber with paper disc insert (34.1 ± 19.8)>Small Finn Chamber (29.8 ± 9.0)> Large Finn Chamber (23.1 ± 7.3)> AL‐Test Chamber (8.0 ± 0.8). Thus, the choice of patch system could produce a false negative error if the system inhibits skin absorption, with a subsequent skin toxicology reaction.