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Sequential immunophenotypic study of lymphoid infiltrate in allergic and irritant reactions
Author(s) -
Avnstorp C.,
Ralfkiaer E.,
Jørgensen J.,
Wantzin G. Lange
Publication year - 1987
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1987.tb01445.x
Subject(s) - antigen , transferrin receptor , immunology , immune system , pathology , macrophage , lymphatic system , chemistry , receptor , medicine , in vitro , biochemistry
Sequential biopsies (4–72 h) of early allergic and irritant patch test reactions have been examined immunohistologically for reactivity with 19 monoclonal antibodies against antigens on lymphoid cells in order to investigate the nature/origin of the infiltrating lymphoid cells and assess their state of activation/proliferation. The composition of the infiltrates was similar in allergic and irritant reactions and consisted of T‐lymphocytes of helper/inducer types in association with T‐cell accessory cells, i.e., Langerhans cells and HLA‐DR‐positive macrophages. No differences in expression of T‐cell or macrophage associated antigens were seen in early as opposed to late biopsies. In contrast, the proportion of cells positive for markers associated with activation (interleukin‐2 receptor) or proliferation (transferrin receptor, the Ki‐67 nuclear antigen) of lymphoid cells was found to increase with time in both types of reaction. These data substantiate the view that T‐cell immune reactions are implicated in both allergic and toxic patch test lesions, and indicate that the lymphocytes in the infiltrates are activated and proliferate.