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Animal model for assessment of skin irritancy
Author(s) -
Anderson Chris,
Sundberg Karin,
Groth Ove
Publication year - 1986
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1986.tb01315.x
Subject(s) - chemistry , irritant contact dermatitis , solvent , irritation , contact dermatitis , dermatology , organic chemistry , allergy , medicine , immunology
Irritant skin reactions from repeated open applications of low concentrations of sodium lauryl sulphate (SLS) have been studied microscopically and microscopically in guinea pigs. After 3 applications daily for 3 days. 2% SLS aqueous solutions gave a naked eye assessment, increase in epidermal thickness and total dermal inflammatory cell response, which was greater than for a 1% SLS solution. The dermal inflammatory cell response was mainly mononuclear (lymphocytic) in nature. With the SLS reactions as control, various organic solvents were studied and ranked against the SLS reactions and internally. Trichlorethylene was the most irritant solvent, ranking as high as 2% SLS Other chlorinated hydrocarbons and aromatic hydrocarbons tested caused irritant reactions. The alcohols and acetone gave no reaction. White spirit was us irritant as trichlorethylene. Thinners were less irritant, around the level of the 1% SLS control reaction. The 4‐day experimental design is a convenient and suitable animal model for screening irritant potential, and provides information relevant to the pathogenesis of irritant contact reactions