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Induction in the guinea pig of delayed hypersensitivity to compound 48/80
Author(s) -
Reller Herbert H.,
Bybee Paul M.
Publication year - 1985
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1985.tb02525.x
Subject(s) - guinea pig , adjuvant , toxicity , delayed hypersensitivity , medicine , hypersensitivity reaction , ethanol , acute toxicity , allergy , pharmacology , immunology , chemistry , immune system , biochemistry
Intradermal injection of compound 48/80 into the nuchal area of the guinea pig in an adjuvant/water emulsion induced strong delayed hypersensitivity Groups of 8 animals were injected weekly fur 1 weeks with 100 μl of 10%, 1%, 0.1% or 0% of compound 48/80 in the adjuvant/water emulsion. 6 of 8 animals in the highest concentration group died between 1 and 24 after the first injection The other concentrations produced no visible evidence of acute toxicity. 2 weeks after the lust injection, the animals were challenged with an open topical application of 1%, 0.1%. 0.01%, and 0% of compound 48/80 in m 3:7 propylene glycol/ethanol. The strongest reactions were found in the 0.1% induction group. Some degree of immunologic tolerance may have occurred in the 1% induction group resulting in somewhat weaker challenge responses.