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The sensitization potential of D & C Yellow No. 11 in guinea pigs
Author(s) -
Palazzolo Matthew J.,
DiPasquale Louis C.
Publication year - 1983
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1983.tb04430.x
Subject(s) - sensitization , medicine , adjuvant , guinea pig , intradermal injection , antigen , delayed hypersensitivity , pharmacology , immunology , chemistry
The certified drug and cosmetic dye substance known as D & C Yellow No. 11 [2‐(2‐quinolyl)‐1,3 indandione] was tested for its dermal sensitization potential in guinea pigs. An induction regimen consisting of a single footpad injection of Complete Freund's Adjuvant diluted with antigen, and an intradermal injection of antigen, produced delayed‐type hypersensitivity reactions. A high sensitization frequency was observed with 50 μg D & C Yellow No. 11 and the positive control 2,4‐dinitrochlorobenzene (DNCB). The reaction severities of both DNCB and 50 μg D & C Yellow No. 11 were comparable, A dose‐response profile was obtained with D & C Yellow No. 11 employing several concentrations (50, 250 and 500 μg/ml). Histologic examination of selected skin sites demonstrated a celluar inflammatory response which was consistent with delayed‐type hypersensitivity.