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A modified technique of guinea pig testing to identify delayed hypersensitivity allergens
Author(s) -
Sato Yoshihisa,
Katsumura Yoshio,
Ichikawa Hidkyuki,
Kobayashi Toshiaki,
Kozuka Takehiio,
Morikawa Fujio,
Ohta Saburo
Publication year - 1981
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1981.tb04058.x
Subject(s) - guinea pig , sensitization , medicine , intradermal injection , adjuvant , delayed hypersensitivity , allergen , immunology , allergy , immune system
A modified guinea pig testing technique was developed For the detection of weak allergens and allergenicity of materials unsuitable for testing by intradermal injection. This test involved the use of Freund's complete adjuvant to stimulate the immune system of the animal, and external application instead of intradermal injection of the test compound in the induction stage. The allergenicity of Sudan III, Brilliant Lake Red R and Sudan I was tested by this procedure. In the dose‐effect study of Sudan I, the dose dependency of a positive reaction of the induction and challenge concentrations was recognised. The test was compared with three other guinea pig sensitization tests. The results obtained with this test correlated well with those obtained with the guinea pig maximization test.