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Histologic aspects of patch test reactions in allergic contact dermatitis
Author(s) -
Rantuccio F.,
Sinisi D.,
Scardigno A.,
Conte A.
Publication year - 1978
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1978.tb03846.x
Subject(s) - patch test , giemsa stain , allergic contact dermatitis , medicine , contact dermatitis , dermatology , allergy , allergen , pathology , immunology
Twenty subjects with allergic contact dermatitis were patch tested with the responsible allergen appliedtofour fixed points on the back. Skin biopsies of the four sites were obtained after 6, 12, 24 and 48 hours, fixed in neutral formalin and twined with hematoxylin‐eosin, Giemsa and toluidine hint. Sections were examined and lymphomonocytes, mast cells, eosinophils and basophils counted. In all cases the infiltrate consisted mainly of lymphomonocytes (80–82 %); mast cells (5 %) and basophils (0.2 %) do not present clear variations within the given time. Eosinophils showed a definite numerical increase in six cases out of 20.