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Periodontal pathogen load and increased antibody response to heat shock protein 60 in patients with cardiovascular disease
Author(s) -
Leishman Shaneen J.,
Ford Pauline J.,
Do Hong Lien,
Palmer Janet E.,
Heng Nicholas C.K.,
West Malcolm J.,
Seymour Gregory J.,
Cullinan Mary P.
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2012.01934.x
Subject(s) - tannerella forsythia , periodontal pathogen , porphyromonas gingivalis , aggregatibacter actinomycetemcomitans , forsythia , medicine , pathogen , fusobacterium nucleatum , antibody , groel , immunology , microbiology and biotechnology , periodontitis , biology , pathology , honeysuckle , biochemistry , alternative medicine , traditional chinese medicine , escherichia coli , gene
Aim To determine the relationship between periodontal pathogen load and anti‐human heat shock protein 60 (h HSP 60) antibodies in patients with established cardiovascular disease ( CVD ). Materials and Methods Participants were cardiovascular patients ( n = 74) with a previous hospital admission for myocardial infarction. Concurrent periodontal pathogen load of Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia and Aggregatibacter actinomycetemcomitans was determined using quantitative real‐time PCR. Serum antibodies to these pathogens, GroEL and h HSP 60 were determined using an ELISA. Results There was a trend for increasing anti‐h HSP 60 antibody as the number of bacterial species increased. The strongest positive correlations were found between anti‐h HSP 60 levels and numbers of T. forsythia ( r = 0.43; p < 0.001) and between anti‐h HSP 60 and anti‐GroEL levels ( r = 0.39; p = 0.001). Patients with extensive periodontal pocketing (≥4 mm) had higher numbers of P. gingivalis and T. forsythia ( p < 0.05) and a higher subgingival pathogen load ( p < 0.05) than patients with minimal pocketing (≤1 site ≥ 4 mm). They also had significantly elevated anti‐h HSP 60 levels ( p < 0.05). Overall, the highest anti‐h HSP 60 levels were seen in patients with extensive periodontal pocketing and all four bacterial species. Conclusions In cardiovascular patients, a greater burden of subgingival infection with increased levels of P. gingivalis and T. forsythia is associated with modestly higher anti‐h HSP 60 levels.