Premium
Antimicrobial responses of primary gingival cells to Porphyromonas gingivalis
Author(s) -
Dommisch Henrik,
Reinartz Markus,
Backhaus Tim,
Deschner James,
Chung Whasun,
Jepsen Søren
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2012.01933.x
Subject(s) - porphyromonas gingivalis , antimicrobial , medicine , microbiology and biotechnology , periodontitis , gingivitis , primary (astronomy) , dentistry , biology , physics , astronomy
Background Human beta‐defensins ( hBD s) and the C – C chemokine ligand 20 ( CCL 20) produced by gingival epithelial cells ( GEC s) and fibroblasts ( HGF s) are antimicrobial peptides ( AMP s) that play an important role in innate immunity. The aim of this study was to determine the differential immune response of GEC s and HGF s to the oral commensal S treptococcus gordonii ( SG ) and the pathogen P orphyromonas gingivalis ( PG ). Material and Methods In addition to the analysis of gingival biopsies, primary GEC s and HGF s were exposed to SG and/or PG , and expression of various AMP s and pro‐inflammatory mediators was studied by real‐time PCR and ELISA . Results Gene expression of AMP s was detected in gingival connective tissue. Both SG and PG induced the mRNA ‐expression of hBD ‐2 and hBD ‐3 in GEC s as well as HGF s after 24 h ( p < 0.05). In HGF s, the commensal bacterium SG stimulated the mRNA s of hBD ‐3 and CCL 20 after 24 h ( p < 0.05), while not in GEC s. In GEC s, the inductive effect of PG on the mRNA ‐expression of hBD ‐2 was amplified when cells were first exposed to commensal SG (for 1 h) prior to stimulation with PG ( SG ‐ PG ; p < 0.05). Conclusion Our data indicate that cell‐bacteria interactions and/or bacteria‐bacteria cross‐talk may have an impact on AMP ‐regulation in gingiva.