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Characterization and immunosuppressive properties of mesenchymal stem cells from periapical lesions
Author(s) -
Đokić Jelena,
Tomić Sergej,
Cerović Snežana,
Todorović Vera,
Rudolf Rebeka,
Čolić Miodrag
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2012.01917.x
Subject(s) - mesenchymal stem cell , peripheral blood mononuclear cell , stem cell , dental pulp stem cells , chemistry , in vitro , collagenase , dental follicle , proinflammatory cytokine , microbiology and biotechnology , immunology , cancer research , inflammation , biology , biochemistry , enzyme
Aim Mesenchymal stem cells ( MSC s) isolated from healthy dental tissues are being investigated as an alternative source of MSC s for the treatment of damaged tissues and inflammatory diseases. Here we investigated whether MSC s from periapical lesions ( PL ‐ MSC s) also possess multi‐lineage differentiation capacity and immunomodulatory properties. Material & Methods PL ‐ MSC s, isolated by collagenase/ DNA se digestion from surgically extracted PL s, were compared with MSC s from non‐inflamed dental pulp ( DP ‐ MSC s) and dental follicle ( DF ‐ MSC s) for their phenotype and multi‐potent differentiation potential. The anti‐inflammatory and immunomodulatory effects of PL ‐ MSC s were studied in co‐culture with peripheral blood mononuclear cells ( PB ‐ MNC s) and PL ‐inflammatory cells ( PL ‐ IC s). Results PL ‐ MSC s were characterized by typical MSC s phenotype, lower clonogenicity and self‐renewal rate, compared to DF ‐ MSC s and DP ‐ MSC s. These cells possess the potential to differentiate into adipocyte‐, osteoblast‐ and chondrocyte‐like cells in vitro, which differs from that of DP ‐ MSC s and DF ‐ MSC s. PL ‐ MSC s inhibited phytohemaglutinine‐induced proliferation of PB ‐ MNC s and production of IL ‐2, IFN γ and IL ‐5 in the co‐culture, probably via TGF ‐β‐dependent mechanisms. These cells also suppressed the production of IL ‐1β, IL ‐6, and TNF ‐α by PL ‐ IC s via soluble mediators, whereas the suppression of IL ‐8 production required a direct cell‐to‐cell contact. Conclusion The differentiation potential of PL ‐ MSC s and their immunosuppressive/anti‐inflammatory properties could be beneficial for the treatment of chronic periodontal diseases.