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The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti‐citrullinated protein antibody) formation
Author(s) -
Nesse Willem,
Westra Johanna,
Wal Jacqueline E.,
Abbas Frank,
Nicholas Anthony P.,
Vissink Arjan,
Brouwer Elisabeth
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2012.01885.x
Subject(s) - periodontitis , citrullination , medicine , periodontium , junctional epithelium , pathology , inflammation , rheumatoid arthritis , connective tissue , antibody , immunology , chemistry , citrulline , dentistry , biochemistry , amino acid , arginine
Aim To determine the presence and location (stroma versus epithelium) of citrullinated proteins in periodontitis tissue as compared to non‐periodontitis tissue and synovial tissue of RA patients. Materials & Methods Periodontitis, healthy periodontal and RA ‐affected synovial tissue samples were collected in addition to buccal swabs. These samples were stained for the presence of citrullinated proteins using polyclonal ( A b5612) and monoclonal ( F 95) antibodies. Furthermore, W estern blotting with F 95 was performed on lysates prepared from periodontal and synovial tissues. Results In periodontitis stroma, increased citrullinated protein presence (80%) was observed compared with control stroma (33%), the latter was associated with inflammation of non‐periodontitis origin. Periodontal epithelium always stained positive for A b5612. Noteworthy, only periodontitis‐affected epithelium stained positive for F 95. All buccal mucosal swabs and 3 of 4 synovial tissue samples stained positive for both A b5612 and F 95. Western blotting with F 95 showed presence of similar citrullinated proteins in both periodontitis and RA ‐affected synovial tissue. Conclusion Within the periodontal stroma, citrullination is an inflammation‐depended process. In periodontal epithelium, citrullination is a physiological process. Additional citrullinated proteins are formed in periodontitis, apparently similar to those formed in RA ‐affected synovial tissue. Periodontitis induced citrullination may play a role in the aetiology of rheumatoid arthritis.