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Maturation of alveolar bone following implantation of an rh GDF ‐5/ PLGA composite into 1‐wall intra‐bony defects in dogs: 24‐week histometric observations
Author(s) -
Park JungChul,
Wikesjö Ulf M. E.,
Koo KiTae,
Lee JungSeok,
Kim YongTae,
Pippig Susanne D.,
Bastone Patrizia,
Kim ChangSung,
Kim ChongKwan
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2012.01868.x
Subject(s) - dental alveolus , plga , beagle , premolar , medicine , dentistry , resorption , ankylosis , bone healing , growth differentiation factor , anatomy , bone morphogenetic protein , pathology , biology , molar , in vitro , biochemistry , gene
Objective The aim of this study was to evaluate long‐term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor‐5 (rh GDF ‐5) in an injectable poly‐lactide‐co‐glycolide‐acid ( PLGA ) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1‐wall, critical‐size, intra‐bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split‐mouth design; defect sites in the same jaw quadrant getting the same treatment) rh GDF ‐5/ PLGA high dose (188 μ g/defect) versus sham‐surgery control (5 animals), and rh GDF ‐5/ PLGA low dose (37 μ g/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24‐week healing interval. Results Clinical healing was uneventful. The rh GDF ‐5 high dose significantly increased bone formation compared with controls in terms of bone area ( p < 0.05), and a high degree of bone maturation was observed in the rh GDF ‐5/ PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rh GDF ‐5/ PLGA appears to support alveolar bone healing/regeneration and the rh GDF ‐5/ PLGA high dose uniquely increased maturation of the regenerated bone.