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Maturation of periodontal tissues following implantation of rh GDF ‐5/β‐ TCP in one‐wall intra‐bony defects in dogs: 24‐week histological observations
Author(s) -
Lee JungSeok,
Wikesjö Ulf M. E.,
Park JungChul,
Jang YongJu,
Pippig Susanne D.,
Bastone Patrizia,
Choi SeongHo,
Kim ChongKwan
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2012.01862.x
Subject(s) - cementum , periodontal fiber , beagle , premolar , medicine , dental alveolus , dentistry , periodontium , regeneration (biology) , dental cementum , anatomy , molar , biology , dentin , microbiology and biotechnology
Abstract Objective Although a previous study reported that recombinant human growth/differentiation factor‐5 (rh GDF ‐5) coated onto a β‐tricalciumphosphate (β‐ TCP ) significantly enhanced periodontal regeneration, the long‐term stability/maturation of the regenerated tissues has not been demonstrated. The objective of this study was to evaluate periodontal regeneration/maturation following application of rh GDF ‐5/β‐ TCP using an established periodontal defect model and a 24‐week healing interval. Material & Methods Unilateral, surgically created, 4 × 4 × 5 mm (length × width × height), one‐wall, critical‐size, intra‐bony periodontal defects at the mandibular second and fourth premolar teeth in five young adult Beagle dogs received rh GDF ‐5/β‐ TCP . Bilateral sites at the fourth premolar in the other four dogs served as pristine controls receiving mucogingival flap surgery without defect induction. The animals were euthanized at 24 weeks for histological analysis. Unpublished data from the previous 8‐week study were used to compare tissue maturation between 8 and 24 weeks. Results Linear histometric observations of cementum and alveolar regeneration showed no significant differences between the 8‐ and 24‐week observation intervals. However, parameters of periodontal tissue maturation showed significant differences between the observation intervals including increased fraction mineralized tissue and lamellar bone ( p < 0.05) and decreased osteocyte counts ( p < 0.05) at 24 weeks compared with 8 weeks. Although the count inserting Sharpey's fibre did not significantly change, regenerated cementum remote from the intact periodontal ligament appeared more highly mineralized and thicker at 24 weeks compared with 8 weeks, and compared with the pristine cementum. Minimal β‐ TCP remained. Conclusions These 24‐week observations suggest that regenerated periodontal tissues in sites receiving rh GDF ‐5/β‐ TCP undergo progressive maturation without debilitating aberrant tissue reactions.