Premium
Detection of Fusobacterium nucleatum in chorionic tissues of high‐risk pregnant women
Author(s) -
Tateishi Fumi,
HasegawaNakamura Kozue,
Nakamura Toshiaki,
Oogai Yuichi,
Komatsuzawa Hitoshi,
Kawamata Kazuya,
Douchi Tsutomu,
Hatae Masayuki,
Noguchi Kazuyuki
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2012.01855.x
Subject(s) - fusobacterium nucleatum , chorionic villi , lipopolysaccharide , medicine , endocrinology , pregnancy , placenta , andrology , biology , fetus , porphyromonas gingivalis , periodontitis , genetics
Aim This study was undertaken to investigate the existence of a periodontopathic bacterium, Fusobacterium nucleatum , in chorionic tissues of pregnant women, and the effects of F. nucleatum on human chorion‐derived cells. Materials and Methods Oral and chorionic tissue samples were collected from 24 high‐risk pregnant women and 15 normal pregnant women. The presence of F. nucleatum in the samples was detected using polymerase chain reaction. Chorion‐derived cells and Toll‐like receptor ( TLR )‐2 or TLR ‐4 gene‐silenced chorion‐derived cells were stimulated with F. nucleatum lipopolysaccharide ( LPS ). Interleukin ( IL )‐6 and corticotrophin‐releasing hormone ( CRH ) levels in the culture supernatants were measured using ELISA . Results F. nucleatum was detected in all oral samples and seven chorionic tissues from the high‐risk pregnant women, but was not detected in chorionic tissues from the normal pregnant women. F. nucleatum LPS significantly increased IL ‐6 and CRH secretion by chorion‐derived cells. The F. nucleatum LPS ‐induced IL ‐6 and CRH levels were significantly reduced in TLR ‐2 or TLR ‐4 gene‐silenced chorion‐derived cells. Conclusions We suggest that F. nucleatum is detected in chorionic tissues of high‐risk pregnant women, but not in chorionic tissues of normal pregnant women, and that F. nucleatum induces IL ‐6 and CRH production via both TLR ‐2 and TLR ‐4 in chorion‐derived cells.