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A.actinomycetemcomitans ‐induced periodontal disease promotes systemic and local responses in rat periodontium
Author(s) -
Bezerra Beatriz de Brito,
Andriankaja Oelisoa,
Kang Jun,
Pacios Sandra,
Bae Hyung Jin,
Li Yu,
Tsiagbe Vincent,
Schreiner Helen,
Fine Daniel H.,
Graves Dana T.
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2011.01847.x
Subject(s) - connective tissue , lymph , immune system , tumor necrosis factor alpha , porphyromonas gingivalis , junctional epithelium , osteoclast , immunology , medicine , periodontitis , andrology , pathology , receptor
Aim To characterize the histologic and cellular response to A . actinomycetemcomitans ( Aa ) infection. Material & Methods Wistar rats infected with Aa were evaluated for antibody response , oral Aa colonization, loss of attachment, PMN recruitment, TNF ‐α in the junctional epithelium and connective tissue, osteoclasts and adaptive immune response in local lymph nodes at baseline and 4, 5 or 6 weeks after infection. Some groups were given antibacterial treatment at 4 weeks. Results An antibody response against Aa occurred within 4 weeks of infection, and 78% of inoculated rats had detectable Aa in the oral cavity ( p < 0.05). Aa infection significantly increased loss of attachment that was reversed by antibacterial treatment ( p < 0.05). TNF ‐α expression in the junctional epithelium followed the same pattern. Aa stimulated high osteoclast formation and TNF ‐α expression in the connective tissue ( p < 0.05). PMN recruitment significantly increased after Aa infection ( p < 0.05). Aa also increased the number of CD 8 + T cells ( p < 0.05), but not CD4 + T cells or regulatory T cells (Tregs) ( p > 0.05). Conclusion Aa infection stimulated a local response that increased numbers of PMN s and TNF ‐α expression in the junctional epithelium and loss of attachment. Both TNF ‐α expression in JE and loss of attachment was reversed by antibiotic treatment. Aa infection also increased TNF ‐α in the connective tissue, osteoclast numbers and CD 8 + T cells in lymph nodes. The results link Aa infection with important characteristics of periodontal destruction.