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Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects
Author(s) -
Oliveira Ana Paula,
Faveri Marcelo,
Gursky Lauren Christine,
Mestnik Maria Josefa,
Feres Magda,
Haffajee Anne D.,
Socransky Sigmund S.,
Teles Ricardo Palmier
Publication year - 2012
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2011.01817.x
Subject(s) - aggressive periodontitis , medicine , scaling and root planing , periodontitis , cytokine , chronic periodontitis , proinflammatory cytokine , pathogenesis , metronidazole , gastroenterology , dentistry , inflammation , antibiotics , biology , microbiology and biotechnology
Aim To examine changes in levels of gingival crevicular fluid ( GCF ) cytokines, after periodontal therapy of generalized aggressive periodontitis ( GAgP ). Materials and Methods Twenty‐five periodontally healthy and 24 GAgP subjects had periodontal clinical parameters measured and gingival crevicular fluid ( GCF ) samples collected from up to 14 sites/subject. GCF samples were analysed using multiplex bead immunoassay for: GM ‐ CSF , IFN ‐γ, IL ‐10, IL ‐1β, IL ‐2, IL ‐6 and TNF ‐α. Aggressive periodontitis subjects were randomly assigned to either scaling and root planing ( SRP ) alone or SRP plus systemic amoxicillin (500 mg) and metronidazole (400 mg) 3 times a day for 14 days. Clinical parameters and GCF cytokines were re‐measured 6 months after treatment. Differences over time were analysed using the W ilcoxon test and between groups using the M ann‐ W hitney test. Results Significant reductions in GCF GM ‐ CSF , IL ‐1β and the ratio IL ‐1β/ IL ‐10 and increases in GCF IL ‐6 were detected after therapy. The mean change in GCF cytokines did not differ significantly between groups. Conclusions Periodontal therapy improved GCF cytokine profiles by lowering IL ‐1β and increasing IL ‐10 levels. The reduction in GCF GM ‐ CSF after therapy implicates this cytokine in the pathogenesis of GA gP. There was no difference between therapies in changes of GCF cytokines.