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MMP ‐8 ‐799 C > T genetic polymorphism is associated with the susceptibility to chronic and aggressive periodontitis in Taiwanese
Author(s) -
Chou YuHsiang,
Ho YaPing,
Lin YingChu,
Hu KaiFang,
Yang YiHsin,
Ho KunYen,
Wu YiMin,
Hsi Edward,
Tsai ChiCheng
Publication year - 2011
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2011.01798.x
Subject(s) - aggressive periodontitis , genotype , periodontitis , chronic periodontitis , matrix metalloproteinase , allele , polymorphism (computer science) , immunology , restriction fragment length polymorphism , medicine , gastroenterology , biology , genetics , gene
Aim Matrix metalloproteinase ( MMP )‐8 is a protease that degrades numerous extracellular molecules and has been implicated in the pathogenesis of periodontitis. Polymorphism in the MMP ‐8 could affect the susceptibility to disease. Our aim was to evaluate the association between periodontitis and MMP ‐8 ‐799 C > T polymorphism. Material and methods Genomic DNA was obtained from 361 chronic periodontitis patients ( CP ), 96 aggressive periodontitis patients ( AgP ), and 106 periodontally healthy controls ( HC ). MMP ‐8 ‐799 C > T polymorphism was determined using the polymerase chain reaction‐restriction fragment length polymorphism ( PCR ‐ RFLP ). Results The frequencies of genotypes in diseased groups were similar but were significantly different from those in the HC . Multivariate logistic regression analysis with adjustment for age, gender and smoking indicated that increased risks of AgP and CP were associated with the ‐799 T allele (in AgP , adjusted OR = 1.99, p = 0.04; in CP , adjusted OR = 1.87, p = 0.007). To avoid the confounded effect of smoking on MMP ‐8 polymorphism to periodontitis, the analysis was conducted on non‐smokers and the associations were significant. Conclusions These results suggested that non‐smoking T aiwanese with the MMP ‐8 ‐799 T allele were associated with the risks of both CP and AgP . Further studies in other ethnic populations are necessary.