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Receptor activator of nuclear factor kappa B ligand antagonists inhibit tissue inflammation and bone loss in experimental periodontitis
Author(s) -
Yuan Huaiping,
Gupte Rohit,
Zelkha Sami,
Amar Salomon
Publication year - 2011
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2011.01780.x
Subject(s) - rankl , periodontitis , osteoprotegerin , porphyromonas gingivalis , dental alveolus , inflammation , medicine , receptor , bone resorption , connective tissue , cytokine , activator (genetics) , immunology , endocrinology , chemistry , dentistry , pathology
Aim The purpose of this study was to assess the role of anti‐bone resorptive agents and an anti‐inflammatory compound in murine Porphyromonas gingivalis ( P. gingivalis ) ‐ induced periodontitis. Material and Methods Six randomly assigned groups were administered vehicle (saline, control) ( n = 6), P. gingivalis infection only (untreated) ( n = 6), human‐ Fc ( n = 4), K avain ( n = 6), OPG‐Fc ( n = 6) and Receptor activator of nuclear factor‐kappa B ( RANK )‐Fc ( n = 6) intraperitoneally at day 0, 3 and 7. Animals were euthanized on day 10 and subjected to comprehensive histomorphometric analysis. To capture the progress of inflammation, serum samples were collected at days 0, 3, 7 and 10 for levels of pro‐inflammatory cytokines. Results Compared with control group, OPG‐Fc , RANK‐Fc and K avain treatment showed significant bone loss reduction with OPG‐Fc performing better than RANK‐Fc or K avain. Epithelial down‐growth showed significant reduction in treatment groups with OPG‐Fc performing better than RANK‐Fc or K avain. Finally, K avain, OPG‐Fc and RANK‐Fc ‐treated mice displayed reduced inflammatory cell counts and cytokine expression particularly at day 7 postinfection. Conclusions RANKL antagonists and K avain effectively reduced alveolar bone loss in P. gingivalis ‐induced periodontitis in our mice model. Compared with RANK‐Fc , K avain‐treated animals showed milder improvement of bone and connective tissue inflammation. Therapeutic implications in the prevention of periodontal bone loss are discussed.