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Diversity and quantitative analysis of Archaea in aggressive periodontitis and periodontally healthy subjects
Author(s) -
Matarazzo Flavia,
Ribeiro Adriana Costa,
Feres Magda,
Faveri Marcelo,
Mayer Marcia Pinto Alves
Publication year - 2011
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2011.01734.x
Subject(s) - archaea , aggressive periodontitis , biology , chronic periodontitis , periodontitis , microbiology and biotechnology , bacteria , genetics , dentistry , medicine
Matarazzo F, Ribeiro AC, Feres M, Faveri M, Mayer MPA. Diversity and quantitative analysis of Archaea in aggressive periodontitis and periodontally healthy subjects. J Clin Peridontol 2011; doi: 10.1111/j.1600‐051X.2011.01734.x. Abstract Aim: To investigate the diversity, levels and proportions of Archaea in the subgingival biofilm of generalized aggressive periodontitis (GAgP; n =30) and periodontally healthy (PH; n =30) subjects. Materials and methods: Diversity was determined by sequencing archaeal 16S rRNA gene libraries from 20 samples (10/group). The levels and proportions of Archaea were analysed by quantitative PCR (qPCR) in four and two samples/subject in GAgP and PH groups, respectively. Results:Archaea were detected in 27/28 subjects and 68% of the sites of the GAgP group, and in 26/30 subjects and 58.3% sites of the PH group. Methanobrevibacter oralis was found in all 20 samples studied, Methanobacterium curvum/congolense in three GAgP and six PH samples, and Methanosarcina mazeii in four samples from each group. The levels and proportions of Archaea were higher in GAgP than in PH, whereas no differences were observed between the two probing depth category sites from the GAgP group. Conclusion:Archaea were frequently found in subjects with periodontal health and GAgP, especially M. oralis . However, the higher levels and proportions ( Archaea /total prokaryotes) of this domain observed in GAgP in comparison with PH subjects indicate a possible role of some of these microorganisms as an environmental modifier in GAgP.