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Microbial shifts during dental biofilm re‐development in the absence of oral hygiene in periodontal health and disease
Author(s) -
Uzel Naciye G.,
Teles Flavia R.,
Teles Ricardo P.,
Song Xiaoging Q.,
Torresyap Gay,
Socransky Sigmund S.,
Haffajee Anne D.
Publication year - 2011
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2011.01730.x
Subject(s) - biofilm , periodontal disease , oral hygiene , dental plaque , dentistry , medicine , hygiene , disease , microbiology and biotechnology , biology , bacteria , pathology , genetics
Uzel NG, Teles FR, Teles RP, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Microbial shifts during dental biofilm re‐development in the absence of oral hygiene in periodontal health and disease. J Clin Peridontol 2011; doi: 10.1111/j.1600‐051X.2011.01730.x. Abstract Aim: To monitor microbial shifts during dental biofilm re‐development. Materials and methods: Supra‐ and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and immediately after tooth cleaning. Samples were taken again from seven teeth in randomly selected quadrants during 1, 2, 4 and 7 days of no oral hygiene. Samples were analysed using checkerboard DNA–DNA hybridization. Species counts were averaged within subjects at each time point. Significant differences in the counts between healthy and periodontitis subjects were determined using the Mann–Whitney test. Results: The total supra‐ and subgingival counts were significantly higher in periodontitis on entry and reached or exceeded the baseline values after day 2. Supragingival counts of Veillonella parvula, Fusobacterium nucleatum ss vincentii and Neisseria mucosa increased from 2 to 7 days. Subgingival counts were greater for Actinomyces , green and orange complex species. Significant differences between groups in supragingival counts occurred for 17 of 41 species at entry, 0 at day 7; for subgingival plaque, these values were 39/41 taxa at entry, 17/41 at day 7. Conclusions: Supragingival plaque re‐development was similar in periodontitis and health, but subgingival species recolonization was more marked in periodontitis.