Premium
Detection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular‐based diagnostics
Author(s) -
Castillo Diana Marcela,
SánchezBeltrán María Carmen,
Castellanos Jaime Eduardo,
Sanz Ignacio,
MayorgaFayad Isabel,
Sanz Mariano,
Lafaurie Gloria Inés
Publication year - 2011
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2011.01717.x
Subject(s) - tannerella forsythia , aggregatibacter actinomycetemcomitans , microbiology and biotechnology , prevotella intermedia , eikenella corrodens , nested polymerase chain reaction , porphyromonas gingivalis , fusobacterium nucleatum , periodontitis , medicine , periodontal pathogen , anaerobic bacteria , scaling and root planing , chronic periodontitis , polymerase chain reaction , biology , bacteria , dentistry , pathology , gene , honeysuckle , biochemistry , genetics , alternative medicine , traditional chinese medicine
Castillo DM, Sánchez‐Beltrán MC, Castellanos JE, Sanz I, Mayorga‐Fayad I, Sanz M, Lafaurie GI. Detection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular‐based diagnostics. J Clin Periodontol 2011; 38: 418–427. doi: 10.1111/j.1600‐051X.2011.01717.x. Abstract Aim: The aim of this study was to assess the presence of subgingival pathogens in peripheral blood samples from periodontitis patients before and after scaling and root planing (Sc/RP) using nested polymerase chain reaction (nested PCR). Materials and Methods: Peripheral blood samples were obtained from 42 patients with severe generalized chronic or aggressive periodontitis. In each patient, four samples of peripheral blood were drawn at different times: immediately before the Sc/RP procedure; immediately after Sc/RP; 15 and 30 min. post‐Sc/RP. Blood samples were analysed for bacteraemia with anaerobic culturing and nested PCR, using universal bacterial primers that target the 16S‐rRNA gene of most bacteria, subsequently re‐amplified with specific primers to Porphyromonas gingivalis , Aggregatibacter actinomycetemcomitans , Tannerella forsythia , Eikenella corrodens , Campylobacter rectus and Prevotella intermedia , using a modified phenol–chloroform method for DNA extraction. Results: Presence of specific periodontal pathogens in peripheral blood after treatment was detected in 54.8% of the patients, in 47.6% with anaerobic culturing and in 19% with nested PCR. In 16.6%, the periodontal pathogens were detected before Sc/RP. P. gingivalis and A. actynomicetemcomitans were the pathogens most frequently detected in the bloodstream before and after Sc/RP. Conclusions: Nested PCR demonstrated the presence of DNA from periodontal pathogens in blood samples in severe periodontitis patients before, during and after periodontal therapy. The use of these molecular‐based techniques may improve the accuracy from the results obtained by haemoculture.