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A targeted fimA DNA vaccine prevents alveolar bone loss in mice after intra‐nasal administration
Author(s) -
Yu Fei,
Xu QingAn,
Chen Weimin
Publication year - 2011
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2010.01700.x
Subject(s) - porphyromonas gingivalis , immunogenicity , dna vaccination , nasal administration , medicine , immunology , periodontitis , immunization , immune system , dental alveolus , antibody , cytotoxic t cell , biology , biochemistry , dentistry , in vitro
Yu F, Xu Q‐A, Chen W. A targeted fimA DNA vaccine prevents alveolar bone loss in mice after intra‐nasal administration. J Clin Periodontol 2011; 38: 334–340. doi: 10.1111/j.1600‐051X.2011.01700.x. Abstract Aim: To construct a dendritic cell (DC)‐targeted DNA vaccine against FimA of Porphyromonas gingivalis and evaluate the immunogenicity and protection in mice. Materials and Methods: A targeted DNA plasmid pCTLA4‐FimA, which encodes the signal peptide and extracellular regions of mouse cytotoxic T lymphocyte‐associated antigen 4 (CTLA4), the hinge and Fc regions of human Ig γ 1 and FimA of P. gingivalis , was constructed. Mice were immunized with pCTLA4‐FimA, the non‐targeted DNA plasmid pFimA, which contains only fimA gene, or pCI vector intra‐nasally. Serum and saliva antibody responses were detected by enzyme‐linked immunosorbent assay. The protection against P. gingivalis ‐induced periodontitis was evaluated by measuring alveolar bone loss in mice. Results: Mice immunized with pCTLA4‐FimA showed elevated levels of specific serum IgG and salivary IgA antibody responses compared with mice immunized with pFimA ( p <0.01). Both pFimA and pCTLA4‐FimA immunized groups showed significantly lower alveolar bone loss, with the magnitude protection greater in the latter ( p <0.01), compared with the pCI immunized group. Conclusions: The DC‐targeted DNA construct pCTLA4‐FimA enhanced both systemic and mucosal immunity following intra‐nasal immunization. A DNA‐based immunization strategy may be an effective way to attenuate periodontitis induced by P. gingivalis .