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Has the use of molecular methods for the characterization of the human oral microbiome changed our understanding of the role of bacteria in the pathogenesis of periodontal disease?
Author(s) -
Wade William Geoffrey
Publication year - 2011
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2010.01679.x
Subject(s) - periodontal disease , pathogenesis , oral microbiome , microbiome , human microbiome , disease , bacteria , microbiology and biotechnology , human disease , medicine , biology , computational biology , immunology , bioinformatics , dentistry , genetics , pathology
Wade WG. Has the use of molecular methods for the characterization of the human oral microbiome changed our understanding of the role of bacteria in the pathogenesis of periodontal disease?J Clin Periodontol 2011; 38 (Suppl. 11): 7–16. doi: 10.1111/j.1600‐051X.2010.01679.x Abstract Background: Only around half of oral bacteria can be grown in the laboratory using conventional culture methods. Molecular methods based on 16S rRNA gene sequence are now available and are being used to characterize the periodontal microbiota in its entirety. Aim: This review describes the cultural characterization of the oral and periodontal microbiotas and explores the influence of the additional data now available from culture‐independent molecular analyses on current thinking on the role of bacteria in periodontitis. Results: Culture‐independent molecular analysis of the periodontal microbiota has shown it to be far more diverse than previously thought. A number of species including some that have yet to be cultured are as strongly associated with disease as those organisms traditionally regarded as periodontal pathogens. Sequencing of bacterial genomes has revealed a high degree of intra‐specific genetic diversity. Conclusions: The use of molecular methods for the characterization of the periodontal microbiome has greatly expanded the range of bacterial species known to colonize this habitat. Understanding the interactions between the human host and its commensal bacterial community at the functional level is a priority.