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Cytokine levels in sites of chronic periodontitis of poorly controlled and well‐controlled type 2 diabetic subjects
Author(s) -
Santos Vanessa Renata,
Ribeiro Fernanda Vieira,
Lima Jadson Almeida,
Napimoga Marcelo Henrique,
Bastos Marta Ferreira,
Duarte Poliana Mendes
Publication year - 2010
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2010.01624.x
Subject(s) - medicine , chronic periodontitis , periodontitis , gastroenterology , cytokine , interleukin
Santos VR, Ribeiro FV, Lima JA, Napimoga MH, Bastos MF, Duarte PM: Cytokine levels in sites of chronic periodontitis of poorly controlled and well‐controlled type 2 diabetic subjects. J Clin Periodontol 2010; doi: 10.1111/j.1600‐051X.2010.01624.x . Abstract Aim: This study compared the levels of tumour necrosis factor (TNF)‐ α , interferon (IFN)‐ γ , interleukin (IL)‐4, IL‐17 and IL‐23 in the gingival crevicular fluid (GCF) from well‐controlled and poorly controlled type 2 diabetic subjects with chronic periodontitis, before and after periodontal therapy. Material and Methods: Eighteen well‐controlled (glycated haemoglobin levels 8%) and 20 poorly controlled (glycated haemoglobin levels >8%) diabetic subjects were enrolled in this study. All subjects were submitted to non‐surgical periodontal therapy. GCF sampling and clinical periodontal parameters were assessed before, 3 and 6 months post‐therapy. Total amounts and concentrations of TNF‐ α , IFN‐ γ , IL‐4, IL‐17 and IL‐23 in the GCF were analysed by enzyme‐linked immunosorbent assay (ELISA). Results: The levels of IL‐17 were higher in poorly than in well‐controlled subjects ( p <0.05), whereas the levels of IFN‐ γ were increased in well‐ compared with poorly controlled subjects at all experimental groups ( p <0.05). In addition, IL‐4 levels were lower in well‐ than poorly controlled diabetic subjects at baseline ( p <0.05). There were no differences between groups for TNF‐ α and IL‐23 at any time points ( p >0.05). Conclusion: These results indicate a predominance of pro‐inflammatory T‐helper type 1 (Th1)‐ or Th17‐cytokines in sites of chronic periodontitis from type 2 diabetic subjects, according to their glycaemic control.