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Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor‐5 in a β ‐tricalcium phosphate carrier into one‐wall intrabony defects in dogs
Author(s) -
Lee JungSeok,
Wikesjö Ulf M. E.,
Jung UiWon,
Choi SeongHo,
Pippig Susanne,
Siedler Michael,
Kim ChongKwan
Publication year - 2010
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2010.01544.x
Subject(s) - cementum , periodontal fiber , regeneration (biology) , wound healing , medicine , dentistry , connective tissue , bone healing , beagle , dental cementum , molar , anatomy , pathology , surgery , biology , dentin , microbiology and biotechnology
Lee J‐S, Wikesjö UME, Jung U‐W, Choi S‐H, Pippig S, Siedler M, Kim C‐K. Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor‐5 in a β ‐tricalcium phosphate carrier into one‐wall intrabony defects in dogs. J Clin Periodontol 2010; 37: 382–389. doi: 10.1111/j.1600‐051X.2010.01544.x Abstract Objective: Recombinant human growth/differentiation factor‐5 (rhGDF‐5) is being evaluated as a candidate therapy in support of periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following the application of rhGDF‐5 on a particulate β ‐tricalcium phosphate ( β ‐TCP) carrier using an established defect model. Materials and Methods: Bilateral 4 × 5 mm (width × depth), one‐wall, critical‐size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre‐molar teeth in 15 Beagle dogs. Unilateral defects in five animals received rhGDF‐5/ β ‐TCP (Scil Technology GmbH); five animals received β ‐TCP solo; and five animals served as sham‐surgery controls. Contralateral sites received treatments reported elsewhere. The animals were sacrificed following an 8‐week healing interval for histological examination. Results: Clinical healing was generally uneventful. Sites implanted with rhGDF‐5/ β ‐TCP exhibited greater enhanced cementum and bone formation compared with β ‐TCP and sham‐surgery controls; cementum regeneration averaged (± SD) 3.83 ± 0.73 versus 1.65 ± 0.82 and 2.48 ± 1.28 mm for the controls ( p <0.05). Corresponding values for bone regeneration height averaged 3.26 ± 0.30 versus 1.70 ± 0.66 and 1.68 ± 0.49 mm ( p <0.05), and bone area 10.45 ± 2.26 versus 6.31 ± 2.41 and 3.00 ± 1.97 mm 2 ( p <0.05). Cementum regeneration included cellular/acellular cementum with or without a functionally oriented periodontal ligament. A non‐specific connective tissue attachment was evident in the sham‐surgery control. Controls exhibited mostly woven bone with primary osteons, whereas rhGDF‐5/ β ‐TCP sites showed a noticeable extent of lamellar bone. Sites receiving rhGDF‐5/ β ‐TCP or β ‐TCP showed some residual β ‐TCP granules apparently undergoing biodegradation without obvious differences between the sites. Sites receiving β ‐TCP alone commonly showed residual β ‐TCP granules sequestered in the connective tissue or fibrovascular marrow. Conclusion: rhGDF‐5/ β ‐TCP has a greater potential to support the regeneration of the periodontal attachment. Long‐term studies are necessary to confirm the uneventful maturation of the regenerated tissues.