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Ex vivo bone morphogenetic protein‐2 gene delivery using gingival fibroblasts promotes bone regeneration in rats
Author(s) -
Shin JoongHo,
Kim KyoungHwa,
Kim SuHwan,
Koo KiTae,
Kim TaeIl,
Seol YangJo,
Ku Young,
Rhyu InChul,
Chung ChongPyoung,
Lee YongMoo
Publication year - 2010
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2009.01522.x
Subject(s) - ex vivo , bone morphogenetic protein 2 , regeneration (biology) , bone morphogenetic protein , bone morphogenetic protein 7 , in vivo , matrix (chemical analysis) , wound healing , gene delivery , bone healing , extracellular matrix , pathology , chemistry , microbiology and biotechnology , anatomy , medicine , genetic enhancement , in vitro , biology , immunology , gene , biochemistry , chromatography
Shin J‐H, Kim K‐H, Kim S‐H, Koo K‐T, Kim T‐I, Seol Y‐J, Ku Y, Rhyu I‐C, Chung C‐P, Lee Y‐M . Ex vivo bone morphogenetic protein‐2 gene delivery using gingival fibroblasts promotes bone regeneration in rats. J Clin Periodontol 2009; 37: 305–311. doi: 10.1111/j.1600‐051X.2009.01522.x . Abstract Aim: The aim of the present study was to investigate bone regeneration following ex vivo bone morphogenetic protein‐2 (BMP‐2) gene delivery using human gingival fibroblasts (HGFs) in rat calvarial defects. Materials and Methods: An 8 mm craniotomy defect was created in Sprague–Dawley rats. The animals were divided into four groups: (1) non‐grafted group, the defect was left empty; (2) collagen matrix group, the defect was filled with collagen matrix only; (3) HGF group, the defect was filled with non‐transduced HGFs on collagen matrix; (4) BMP‐2/HGF group, the defect was filled with BMP‐2 gene‐transduced HGFs on collagen matrix. Animals were sacrificed at 2 and 4 weeks after surgery, and micro‐computed tomographic and histologic observations were performed. Results: The BMP‐2/HGF group showed promoted osseous healing of calvarial defects, as compared with the other groups. At both 2 and 4 weeks, regenerated bone area was significantly greater in the BMP‐2/HGF group than the other three groups. Quite a few number of transplanted HGFs were observed within the regenerated bone tissues. Conclusions: The results of this study suggest that ex vivo BMP‐2 gene delivery induces prominent bone regeneration in vivo and HGFs may be useful as target cells for ex vivo gene therapy.