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The interplay of lipopolysaccharide‐binding protein and cytokines in periodontal health and disease
Author(s) -
Ren Lei,
Jiang Zi Qiang,
Fu Yun,
Leung Wai Keung,
Jin Lijian
Publication year - 2009
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2009.01436.x
Subject(s) - cd14 , lipopolysaccharide binding protein , lipopolysaccharide , periodontitis , pathogenesis , medicine , chronic periodontitis , immunology , interleukin , immunohistochemistry , inflammation , connective tissue , cytokine , pathology , immune system
Aim: Periodontal pathogenesis is characterized by Gram‐negative bacteria activation of series of pro‐ and anti‐inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS‐binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)‐1 β and IL‐10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL‐1 β and IL‐10 by human gingival fibroblasts (HGF). Material and Methods: Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL‐1 β and IL‐10 was detected by immunohistochemistry. The mRNA expression of IL‐1 β and IL‐10 in HGF was detected by RT‐PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme‐linked immunosorbent assay. Results: IL‐1 β was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL‐10 was mainly expressed in the intercellular spaces of connective tissues. IL‐1 β displayed a reverse pattern of expression levels with reference to IL‐10, and a negative correlation existed between LBP and the ratio of IL‐1 β /IL‐10. rhLBP suppressed E. coli LPS‐induced IL‐1 β expression by HGF. Conclusion: An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis.