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Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor‐5 (rhGDF‐5) in an absorbable collagen sponge carrier into one‐wall intrabony defects in dogs: a dose‐range study
Author(s) -
Kim TaeGyun,
Wikesjö Ulf M. E.,
Cho KyooSung,
Chai JungKiu,
Pippig Susanne D.,
Siedler Michael,
Kim ChongKwan
Publication year - 2009
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2009.01420.x
Subject(s) - cementum , ankylosis , growth differentiation factor , dentistry , medicine , regeneration (biology) , histology , anatomy , biology , pathology , bone morphogenetic protein , microbiology and biotechnology , biochemistry , gene , dentin
Aim: Recombinant human growth/differentiation factor‐5 (rhGDF‐5) is being evaluated as a candidate therapy in support of periodontal regeneration. The objective of this study was to evaluate cementum and alveolar bone formation, and aberrant healing events following surgical implantation of rhGDF‐5 in an absorbable collagen sponge (ACS) carrier using an established periodontal defect model. Materials and Methods: Bilateral 4 × 5 mm (width × depth), one‐wall, critical‐size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre‐molar teeth in 15 Beagle dogs. Five animals received 1 μ g/defect and five animals 20 μ g/defect rhGDF‐5 in unilateral defect sites. Contralateral sites received treatments reported elsewhere. Five animals received rhGDF‐5/ACS with 0 (buffer control) and 100 μ g/defect rhGDF‐5 in contralateral defect sites. The animals were euthanized at 8 weeks post‐surgery for histologic and histometric evaluation. Results: Surgical implantation of rhGDF‐5 stimulated significant periodontal regeneration. Cementum formation was significantly enhanced in sites implanted with rhGDF‐5 (1 and 100 μ g) compared with control ( p <0.05). Similarly, bone formation height was significantly greater in sites receiving rhGDF‐5 (1 and 100 μ g) compared with control ( p <0.05). There were no significant or remarkable differences in bone and cementum formation within the selected dose interval (1, 20 and 100 μ g rhGDF‐5). None of the control or the rhGDF‐5 sites exhibited root resorption, ankylosis, or other aberrant tissue reactions. Conclusion: Surgical implantation of rhGDF‐5/ACS may be used safely to support periodontal wound healing/regeneration in intrabony periodontal defects without complications.