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Mouse model of experimental periodontitis induced by Porphyromonas gingivalis / Fusobacterium nucleatum infection: bone loss and host response
Author(s) -
Polak David,
Wilensky Asaf,
Shapira Lior,
Halabi Amal,
Goldstein Dita,
Weiss Ervin I.,
HouriHaddad Yael
Publication year - 2009
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2009.01393.x
Subject(s) - fusobacterium nucleatum , porphyromonas gingivalis , periodontitis , dental alveolus , osteoprotegerin , microbiology and biotechnology , bacteroidaceae , tumor necrosis factor alpha , medicine , oral infection , immunology , fusobacterium , host response , clinical attachment loss , inflammation , lipopolysaccharide , biology , bacteria , bacteroides , immune system , dentistry , receptor , genetics , activator (genetics)
Aim: To compare the effect of oral infection with Porphyromonas gingivalis or Fusobacterium nucleatum versus infection with both bacteria on mouse periodontal tissues, and to characterize the inflammatory response. Materials and Methods: Mice were orally infected with P. gingivalis, F. nucleatum or both. At 42 days post‐infection, alveolar bone loss was quantified using micro‐computerized tomography. Tumour necrosis factor‐ α (TNF‐ α ) and interleukin (IL)‐1 β levels induced by the infection were quantified using the subcutaneous chamber model. Results: Mice orally infected with F. nucleatum / P. gingivalis exhibited significantly more bone loss compared with that of mono‐infected and sham‐infected mice. F. nucleatum / P. gingivalis infection also increased the levels of TNF‐ α and IL1 β compared with the levels found in the mono‐infected groups. Conclusions: Polymicrobial infection with P. gingivalis / F. nucleatum aggravates alveolar bone loss and induces a stronger inflammatory response compared with that observed upon infection with either bacterium alone. The results suggest that oral infection of mice with a mixture of P. gingivalis and F. nucleatum may be superior to mono‐infection models of experimental periodontitis.