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Common single nucleotide polymorphisms in cyclooxygenase‐2 and risk of severe chronic periodontitis in a Chinese population
Author(s) -
Xie ChengJie,
Xiao LiMin,
Fan WeiHua,
Xuan DongYing,
Zhang JinCai
Publication year - 2009
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2008.01366.x
Subject(s) - haplotype , single nucleotide polymorphism , odds ratio , medicine , chronic periodontitis , gastroenterology , genotype , periodontitis , logistic regression , restriction fragment length polymorphism , case control study , biology , genetics , gene
Aim: Several common single nucleotide polymorphisms (SNPs) of the cyclooxygenase‐2 (COX‐2) gene have been reported to be functional. The association between −1195GA, −765GC and 8473TC of COX‐2, and severe chronic periodontitis (CP) in a Chinese population was investigated. Material and Methods: 148 cases of healthy controls (control group) and 146 cases of severe CP were recruited in this study. Genotypes of −1195GA, −765GC and 8473TC were determined by polymerase chain reaction restriction fragment length polymorphism (PCR‐RFLP). The distributions of genotypes and haplotypes were compared by χ 2 test and the odds ratios (ORs) were calculated by logistic regression analysis. Results: The prevalence of the −1195A was more prevalent in CP group (60.62%) than control group (51.35%), and the distributions of the −765C and 8473C were higher in control group (6.76% and 21.96%) compared with CP group (3.08% and 15.07%). Only genotype distribution of −1195GA was significant when p ‐value was corrected for multiple testing ( p c =0.033). The adjusted ORs for the −1195AA/GA, −765GC and 8473CC/TC were 2.49 (95% CI=1.33–4.69, p =0.005), 0.45 (95% CI=0.20–1.04, p =0.061) and 0.67 (95% CI=0.41–1.11, p =0.118). Subjects with the haplotype AGT had a significantly higher risk of periodontitis than those with the most common haplotype GGT (OR=1.91, 95% CI=1.32–2.76, p c <0.001). Conclusions: It suggests the −1195A variant is associated with an increased risk for severe CP.