Premium
Herpes viruses in periodontal compromised sites: comparison between HIV‐positive and ‐negative patients
Author(s) -
Grande Sabrina Rosa,
Imbronito Ana Vitória,
Okuda Osmar Shizuo,
Lotufo Roberto Fraga Moreira,
Magalhães Marina Helena Gallottini,
Nunes Fabio Daumas
Publication year - 2008
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2008.01307.x
Subject(s) - saliva , gingivitis , medicine , cytomegalovirus , herpes simplex virus , periodontitis , human cytomegalovirus , virus , nested polymerase chain reaction , immunology , clinical attachment loss , viral load , herpesviridae , viral disease , polymerase chain reaction , biology , dentistry , biochemistry , gene
Aim: The objective of this study was to compare the frequency of herpes simplex virus type 1 (HSV‐1), Epstein–Barr virus (EBV) and human cytomegalovirus (HCMV) in subgingival plaque, saliva and peripheral blood of HIV‐positive and‐negative patients with periodontal disease. Material and Methods: Fifty HIV‐positive subjects (23 with gingivitis, 27 with periodontitis) and 50 healthy HIV‐negative patients with chronic periodontitis were included in the study. Parameters of probing depth (PD), clinical attachment level (CAL), gingival index and plaque index were recorded. The samples were processed for viral identification by the nested polymerase chain reaction technique. Results: HCMV was the most prevalent virus in HIV‐positive (82%) and‐negative patients (84%), and the detection in the three samples was similar ( p >0.05). HSV‐1 was the least prevalent virus in both groups, being detected in similar frequencies in oral sites and in peripheral blood. EBV‐1 was found more frequently in saliva and subgingival plaque of HIV‐positive patients than in HIV‐negative patients ( p 0.05). Conclusions: EBV‐1 was more frequently recovered in oral sites of HIV‐positive patients than in HIV‐negative patients.