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Improvement of periodontal condition by probiotics with Lactobacillus salivarius WB21: a randomized, double‐blind, placebo‐controlled study
Author(s) -
Shimauchi Hidetoshi,
Mayanagi Gen,
Nakaya Seigo,
Minamibuchi Maiko,
Ito Yasuhiro,
Yamaki Keiko,
Hirata Haruhisa
Publication year - 2008
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2008.01306.x
Subject(s) - lactobacillus salivarius , double blind , medicine , placebo , randomized controlled trial , probiotic , dentistry , lactobacillus , food science , biology , pathology , bacteria , fermentation , alternative medicine , genetics
Aim: This randomized clinical study was designed to evaluate the effect of probiotic intervention using lactobacilli on the periodontal condition of volunteers without severe periodontitis. Material and Methods: Freeze‐dried Lactobacillus salivarius WB21 (WB21)‐containing tablets or a placebo were given to volunteers in a double‐blind randomized study. A total of 66 volunteers were finally enrolled and randomly assigned to receive tablets containing WB21 (6.7 × 10 8 CFU) with xylitol or xylitol alone (placebo) three times a day for 8 weeks. Periodontal clinical parameters and whole saliva samples were obtained at baseline (BL), 4 weeks, and the end of the interventional period (8 weeks). Salivary lactoferrin (Lf) levels were measured by enzyme‐linked immunosorbent assay. Lactobacilli in saliva and plaque samples was detected by semi‐quantitative RT‐PCR using 16S rRNA primers. Results: Periodontal clinical parameters were improved in both groups after an 8‐week intervention. Current smokers in the test group showed a significantly greater improvement of plaque index and probing pocket depth from BL when compared with those in the placebo group. Salivary Lf level was also significantly decreased in the test group smokers. Conclusion: Our results indicate that probiotics could be useful in the improvement/maintenance of oral health in subjects at a high risk of periodontal disease.