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Genetic markers of tumour necrosis factor α in aggressive and chronic periodontitis
Author(s) -
Schulz Susanne,
Machulla Helmut K. G.,
Altermann Wolfgang,
Klapproth Jana,
Zimmermann Uta,
Gläser Christiane,
Kluttig Alexander,
Stein Jamal,
Schaller HansGünter,
Reichert Stefan
Publication year - 2008
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2008.01226.x
Subject(s) - periodontitis , odds ratio , haplotype , chronic periodontitis , single nucleotide polymorphism , aggressive periodontitis , prevotella intermedia , medicine , risk factor , gastroenterology , genotype , biology , immunology , genetics , gene , porphyromonas gingivalis
Abstract Aim: Tumour necrosis factor α (TNF α ) plays an important role in the pathogenesis of periodontitis. TNF α production is influenced by gene polymorphisms. The aim of this study was to evaluate links between genetic variants and chronic/aggressive periodontitis in a multivariate model. Subjects: One hundred and twenty‐three periodontitis patients (chronic: n =54, aggressive: n =69) and 52 healthy controls without periodontitis were included in the study. Material and Methods: Single nucleotide polymorphisms (SNPs) c.−308G>A, c.−238G>A and haplotypes were analysed by a polymerase chain reaction with sequence‐specific primers (PCR‐SSP). The clinical investigation included smoking status, plaque and bleeding indexes, pocket depth and attachment loss. Results:Prevotella intermedia occurred more frequently in individuals positive for the −308GG/−238GG haplotype combination (Odds Ratio=2, 95% Confidence interval: 1.1–3.7, p =0.037, 1− β =61%). In binary logistic regression analyses, this TNF α haplotype could not be shown to be associated with periodontitis considering smoking, age, gender and approximal plaque index or subgingival bacterial colonization as confounding factors. Conclusions: Although the genetic background of TNF α could be shown to be associated with subgingival colonization with P. Intermedia , there is no evidence that it is an independent risk factor for periodontitis in multivariate models.