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Mesenchymal stem cells and platelet‐rich plasma enhance bone formation in sinus grafting: a histomorphometric study in minipigs
Author(s) -
Pieri Francesco,
Lucarelli Enrico,
Corinaldesi Giuseppe,
Iezzi Giovanna,
Piattelli Adriano,
Giardino Roberto,
Bassi Massimo,
Donati Davide,
Marchetti Claudio
Publication year - 2008
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2008.01220.x
Subject(s) - platelet rich plasma , osseointegration , maxillary sinus , mesenchymal stem cell , sinus (botany) , grafting , dental implant , bone grafting , medicine , dentistry , implant , biomedical engineering , surgery , pathology , chemistry , platelet , biology , botany , organic chemistry , genus , polymer
Objectives: Autologous, allogenic, and alloplastic materials for sinus augmentation have specific drawbacks, which has stimulated an ongoing search for new materials and tissue‐engineering constructs. We investigated whether mesenchymal stem cells (MSCs) and platelet‐rich plasma (PRP) seeded on a fluorohydroxyapatite (FH) scaffold can improve bone formation and bone‐to‐implant contact (BIC) in maxillary sinus grafting. Material and Methods: Bilateral sinus augmentation procedures were performed in eight minipigs. MSCs, PRP, and FH scaffold (test site) or FH alone (control site) were grafted in each maxillary sinus. Distal to the osteotomy, one dental implant per sinus was placed in the grafting material through the facial sinus wall. The animals were killed 3 months after grafting, and block sections of the implant sites were harvested and prepared for histomorphometric analysis. Results: After 12 weeks, a significant increase in bone formation occurred in the test sites compared with the control sites (42.51% versus 18.98%; p =0.001). In addition, BIC was significantly greater in the test sites compared with the control sites in the regenerated area (23.71% versus 6.63%; p =0.028). Conclusions: These findings show that sinus augmentation with MSCs–PRP, combined with FH may enhance bone formation and osseointegration of dental implants compared with FH alone in minipigs.