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Polymorphisms in the 5′ flanking region of IL12RB2 are associated with susceptibility to periodontal diseases in the Japanese population
Author(s) -
TakeuchiHatanaka Kazu,
Ohyama Hideki,
Nishimura Fusanori,
KatoKogoe Nahoko,
Soga Yoshihiko,
Matsushita Sho,
Nakasho Keiji,
Yamanegi Koji,
Yamada Naoko,
Terada Nobuyuki,
Takashiba Shogo
Publication year - 2008
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2008.01208.x
Subject(s) - immunology , pathogenesis , periodontitis , single nucleotide polymorphism , allele , immune system , aggressive periodontitis , biology , snp , antibody , population , chronic periodontitis , genetics , genotype , medicine , gene , environmental health
Objectives: The expression of interleukin (IL)‐12R β 2 molecule is a crucial regulatory factor in the T‐helper type (Th) 1 differentiation of T cells. To elucidate the role of the cell‐mediated immune (CMI) response in the pathogenesis of periodontitis, Japanese periodontal patients were subjected to single nucleotide polymorphism (SNP) analyses of the 5′ flanking region of IL12RB2 , whose variants are frequently detected in lepromatous leprosy patients, in which the very weak cellular immune response is caused by low expression of IL‐12R β 2. Material and Methods: The gene polymorphisms of the 5′ flanking region of IL12RB2 were examined in subjects with several types of periodontal disease and in healthy controls. Serum immunoglobulin (Ig) G antibody titres against periodontopathic bacteria were measured and compared in periodontal patients with and without variant alleles of IL12RB2 . Results: The frequencies of variant alleles of IL12RB2 were significantly higher in aggressive periodontitis patients as compared with healthy controls or chronic periodontitis patients. Serum IgG titres against all periodontal bacteria examined in subjects carrying variant alleles were higher than those in subjects without variant alleles. Conclusion: IL‐12R β 2 SNPs could be useful as genetic markers to access the susceptibility of the general population to periodontal disease. Low CMI responses or high humoral responses are associated with the pathogenesis of inflammatory periodontal diseases.