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Characterization of progressive periodontal lesions in chronic periodontitis patients: levels of chemokines, cytokines, matrix metalloproteinase‐13, periodontal pathogens and inflammatory cells
Author(s) -
Silva Nora,
Dutzan Nicolas,
Hernandez Marcela,
Dezerega Andrea,
Rivera Oriana,
Aguillon Juan Carlos,
Aravena Octavio,
Lastres Pedro,
Pozo Patricia,
Vernal Rolando,
Gamonal Jorge
Publication year - 2008
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2007.01190.x
Subject(s) - tannerella forsythia , porphyromonas gingivalis , periodontitis , chronic periodontitis , medicine , periodontal pathogen , chemokine , matrix metalloproteinase , immunology , gingivitis , proinflammatory cytokine , inflammation , pathology , dentistry , honeysuckle , alternative medicine , traditional chinese medicine
Background and aims: Periodontitis is an infection with an episodic nature of tissue support destruction. The aim of this work was to determine the levels of chemokines, cytokines, matrix metalloproteinase‐13, periodontal pathogens and inflammatory cells in periodontal sites characterized by active periodontal connective tissue destruction. Material and Method: Fifty‐six patients with moderate or advanced severity of chronic periodontitis were selected. Periodontitis was characterized by at least six sites with probing depth 5 mm, clinical attachment level 3 mm and radiographic bone loss. Periodontitis progression was determined by the tolerance method. Receptor activator for nuclear factor κ B‐ligand (RANK‐L), monocyte chemoattractant protein‐1 (MCP‐1), tumour necrosis factor‐ α (TNF‐ α ), IL‐1 β , MMP‐13, Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis , Tannerella forsithia and inflammatory cells levels were determined. Statistical analysis was performed using the Stata ® 7.0 software. Data were expressed as mean±SD and paired samples t ‐test and χ 2 tests were used. Results: Higher RANK‐L, IL‐1 β and MMP‐13 activity levels were observed in active sites ( p <0.05). The proportion of P. gingivalis , A. actinomycetemcomitans , T. forsythia and the number of CD4 + T were higher in active than in inactive sites ( p >0.05). Conclusion: The detection of periodontopathic bacteria, host matrix metalloproteinases and cytokines in periodontitis patients with lesions undergoing episodic attachment loss could partially explain the mechanisms associated with the destruction of the supporting tissues of the tooth.