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Effects of enamel matrix derivative and transforming growth factor‐ β 1 on human periodontal ligament fibroblasts
Author(s) -
Rodrigues Thaisângela L. S.,
Marchesan Julie T.,
Coletta Ricardo D.,
Novaes Arthur B.,
Grisi Márcio F. de M.,
Souza Sérgio L. S.,
Taba Mário,
Palioto Daniela B.
Publication year - 2007
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2007.01090.x
Subject(s) - enamel matrix derivative , periodontal fiber , transforming growth factor , chemistry , periodontium , cell growth , cell adhesion , fibroblast , wound healing , alkaline phosphatase , matrix (chemical analysis) , cell , in vitro , microbiology and biotechnology , dentistry , regeneration (biology) , medicine , immunology , biochemistry , biology , chromatography , enzyme
Aim: The objective of this study was to evaluate the effects of enamel matrix derivative (EMD), transforming growth factor‐ β 1 (TGF‐ β 1), and a combination of both factors (EMD+TGF‐ β 1) on periodontal ligament (PDL) fibroblasts. Material and methods: Human PDL fibroblasts were obtained from three adult patients with a clinically healthy periodontium, using the explant technique. The effects of EMD, TGF‐ β 1, or a combination of both were analysed on PDL cell proliferation, adhesion, wound healing, and total protein synthesis, and on alkaline phosphatase (ALP) activity and bone‐like nodule formation. Results: Treatment with EMD for 4, 7, and 10 days increased cell proliferation significantly compared with the negative control ( p <0.05). At day 10, EMD and EMD+TGF‐ β 1 showed a higher cell proliferation compared with TGF‐ β 1 ( p <0.01). Cell adhesion was significantly up‐regulated by TGF‐ β 1 compared with EMD and EMD+TGF‐ β 1 ( p <0.01). EMD enhanced in vitro wound healing of PDL cells compared with the other treatments. Total protein synthesis was significantly increased in PDL cells cultured with EMD compared with PDL cells treated with TGF‐ β 1 or EMD+TGF‐ β 1 ( p <0.05). EMD induced ALP activity in PDL fibroblasts, which was associated with an increase of bone‐like nodules. Conclusion: These findings support the hypothesis that EMD and TGF‐ β 1 may play an important role in periodontal regeneration. EMD induced PDL fibroblast proliferation and migration, total protein synthesis, ALP activity, and mineralization, while TGF‐ β 1 increased cellular adhesion. However, the combination of both factors did not positively alter PDL fibroblast behaviour.