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Single‐nucleotide polymorphisms in the IL‐4 and IL‐13 promoter region in aggressive periodontitis
Author(s) -
Gonzales J. R.,
Mann M.,
Stelzig J.,
Bödeker R. H.,
Meyle J.
Publication year - 2007
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2007.01086.x
Subject(s) - genotype , aggressive periodontitis , allele , allele frequency , single nucleotide polymorphism , exact test , genotype frequency , immunology , interleukin , biology , polymorphism (computer science) , medicine , genetics , periodontitis , gastroenterology , microbiology and biotechnology , gene , cytokine
IL‐4 and IL‐13 polymorphisms have been shown to influence the susceptibility to systemic diseases. In this study, possible associations between the IL‐4 −590 C→T, IL‐4 −34 C→T, IL‐13 −1112 C→T and IL‐13 −1512 A→C promoter polymorphisms were investigated in subjects with generalized aggressive periodontitis (AgP) compared with healthy individuals. Material and Methods: Fifty‐eight patients with diagnosis of generalized AgP and 51 matched healthy controls participated in the study. Blood samples were collected and DNA isolated. Molecular analyses were performed by PCR‐RFLP in a blind fashion. Genotype and allele frequencies among study groups were compared using Fisher's exact test ( α value: 0.05). Pearson's χ 2 test was used for analysis of Hardy–Weinberg equilibrium. Results: The frequency of the IL‐4 −590 T/T and IL‐4 −34 T/T genotypes differed significantly between groups ( p =0.05, 0.02, respectively), although the allele frequencies were similar. There was a higher frequency of the IL‐4 −590 T/T and IL‐4 −34 T/T genotypes in patients with AgP compared with controls. The genotype and allele frequencies of the IL‐13 polymorphisms did not differ between groups. Conclusions: This study demonstrated an association between the IL‐4 −590 T/T and IL‐4 −34 T/T genotypes and AgP. Further research is necessary to prove if there is an association of these polymorphisms with AgP, and if the polymorphisms have a functional effect.