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Proportion of antibiotic resistance in subgingival plaque samples from Mexican subjects
Author(s) -
AlmaguerFlores Argelia,
MorenoBorjas JazminYunuen,
SalgadoMartinez Araceli,
SanchezReyes MarcoAntonio,
AlcantaraMaruri Eulalio,
XimenezFyvie LaurieAnn
Publication year - 2006
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2006.00975.x
Subject(s) - dentistry , dental plaque , antibiotic resistance , antibiotics , medicine , microbiology and biotechnology , biology
Aim: To determine the proportion of bacteria resistant to amoxicillin and doxycycline in subgingival plaque samples from Mexican subjects. Materials and Methods: Two subgingival plaque samples were taken from 20 Mexican subjects. Samples were dispersed, diluted and plated on non‐antibiotic agar plates and on plates containing 0.5, 1, 2, 4, 8 and 16 μ g/ml of either amoxicillin or doxycycline. The proportion of resistant bacteria was calculated based on the total number of colony‐forming units present in the non‐antibiotic containing plates. Results: On average, 0.4–13.4% and 0.9–20.4% of the total cultivable subgingival microbiota was resistant to the concentrations tested of amoxicillin and doxycycline, respectively. The differences between antibiotics were statistically significant for the 0.5, 2 and 4 μ g/ml concentrations ( p <0.05, Wilcoxon's test). Conclusions: Our findings revealed that a relatively small proportion of the total cultivable subgingival microbiota from Mexican subjects was resistant to amoxicillin and doxycycline.