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Elevated levels of gene expression for collagen and decorin in human gingival overgrowth
Author(s) -
Dannewitz Bettina,
Edrich Christina,
Tomakidi Pascal,
Kohl Annette,
Gabbert Olaf,
Staehle HansJörg,
Steinberg Thorsten
Publication year - 2006
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2006.00937.x
Subject(s) - decorin , gene expression , extracellular matrix , gene , in situ hybridization , messenger rna , iif , microbiology and biotechnology , immunofluorescence , biology , real time polymerase chain reaction , chemistry , biochemistry , immunology , indirect immunofluorescence , antibody , proteoglycan
Objectives: It has been demonstrated that extracellular matrix molecules are involved in cyclosporine‐induced gingival overgrowth (GO). However, for many of these molecules, it remains unclear whether their abundance is modulated on the protein and gene expression level. Material and Methods: To contribute to this clarification, we have analysed the protein and mRNA expression of type‐I collagen (COL1) and decorin (DC) in native specimens obtained from five patients with GO, and matched normal tissue using indirect immunofluorescence (IIM), in situ hybridization (ISH) and quantitative polymerase chain reaction (PCR). Results: IIF revealed a largely co‐localized although remarkably increased abundance for COL1 and DC in GO. This increase coincided with an up‐regulated gene expression observed for both molecules, as detected by ISH and quantitative PCR. Conclusions: Analysis of our data clearly demonstrates elevated levels for COL1 and DC and shows for the first time in native human tissue that involvement of these genes in GO is not confined to the protein level but also includes the transcriptional level.