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Areca nut extracts modulated expression of alkaline phosphatase and receptor activator of nuclear factor κ B ligand in osteoblasts
Author(s) -
Ling LiJane,
Ho FengChuang,
Chen YenTing,
Holborow Douglas W.,
Liu TsungYun,
Hung ShanLing
Publication year - 2005
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2005.00687.x
Subject(s) - osteoprotegerin , rankl , areca , viability assay , alkaline phosphatase , receptor , activator (genetics) , medicine , chemistry , endocrinology , dentistry , microbiology and biotechnology , cell , biochemistry , biology , enzyme , nut , engineering , structural engineering
Objectives: Areca chewers have a higher prevalence of periodontal diseases than non‐chewers. This study was to determine the possible effects of ripe areca nut extracts (rANE) on viability and gene expression of alkaline phosphatase (ALP), receptor activator of nuclear factor‐ κ B ligand (RANKL) and osteoprotegerin (OPG) in human osteoblasts. Methods: The effects of rANE on cell viability of osteoblast‐like MG63 cells were determined using 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide (MTT) that measures metabolic activity. Gene expression of ALP, RANKL and OPG was examined using reverse transcription‐polymerase chain reaction. ALP activity and RANKL protein were further examined using substrate assay and confocal laser scanning microscopy, respectively. Results: Relative viability was reduced to approximately 50% when 25 μg/ml of rANE was used. The expression of OPG mRNA in MG63 cells was not altered by rANE. However, decreased levels of mRNA and enzyme activity of ALP were observed. Moreover, the expressions of mRNA and protein of RANKL were stimulated by rANE in a dose‐dependent manner. Conclusions: The rANE affected morphology and viability of osteoblasts. We also present novel evidence demonstrating that areca nut may compromise the periodontal health of areca chewers by suppression of ALP gene expression and elevation of RANKL gene expression in osteoblasts.