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A model of periodontitis in the rat: effect of lipopolysaccharide on bone resorption, osteoclast activity, and local peptidergic innervation
Author(s) -
Dumitrescu Alexandrina L.,
Abd ElAleem Seham,
MoralesAza Begonia,
Donaldson Lucy F.
Publication year - 2004
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2004.00528.x
Subject(s) - periodontitis , inflammation , junctional epithelium , osteoclast , bone resorption , periodontal fiber , medicine , lipopolysaccharide , fusobacterium nucleatum , pathology , resorption , porphyromonas gingivalis , molar , dentistry , epithelium , receptor
Objective: To establish and characterise a rat model of periodontitis that reiterates the features of human disease. Methods: Periodontal inflammation was induced by a single injection of 10 μ g liposaccharide (LPS) ( Salmonella typhimurium ) in 1 μ l saline into rat mandibular gingiva at the buccomesial aspect of the second molar. Animals were killed after 3, 7 and 10 days, mandibles dissected and sectioned for histological and immunocytochemical analysis. Results: LPS injection resulted in a significant gingival and periodontal inflammation with inflammatory infiltrate, apical migration of the junctional epithelium, interdental bone loss, and activation of osteoclasts at the site of injection 7 and 10 days after injection. At 10 days post injection, there was a significant trend for bone loss on both sides of the mandible. Periodontal inflammation was associated with alteration in the levels of calcitonin gene‐related peptide‐like immunoreactivity in nerve terminals innervating the inflamed gingival papilla. Conclusion: Intragingival injection of LPS in the rat provides an easily induced reproducible experimental model of periodontal inflammation that reiterates features of human disease.