Premium
Periodontal repair in dogs: effect of recombinant human transfornning growth factor‐β 1 on guided tissue regeneration
Author(s) -
Wikesjö Ulf M. E.,
Razi Saghi S.,
Sigurdsson Thorarinn J.,
Tatakis Dimitris N.,
Lee Michael B.,
Ongpipattanakul Boonsri,
Nguyen Tue,
Hardwick Ross
Publication year - 1998
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1998.tb02476.x
Subject(s) - cementum , regeneration (biology) , dental alveolus , beagle , premolar , dentistry , medicine , growth factor , dentin , microbiology and biotechnology , molar , biology , receptor
. This study evaluated alveolar bone and cementum regeneration following surgical implantation of recombinant human transforming growth factor‐ β 1 (rhTGF‐β 1 ) in conjunction with guided tissue regeneration (GTR). Supraalvcolar, critical size, periodontal defects were surgically created around the 3rd and 4th mandibular premolar teeth in right and left jaw quadrants in 5 beagle dogs. Alternate jaw quadrants in consecutive animals received rhTGF‐β 1 in a CaCO 3 / hydroxyethyl starch carrier with GTR, or carrier with GTR alone (control), 20μg of rhTGF‐/A in buffer solution was incorporated into approximately 0.8 ml of carrier for each defect scheduled to receive rhTGF‐β 1 . Animals were sacrificed at week 4 postsurgery and tissue blocks were harvested and processed for histo‐metric analysis. Clinical healing was generally uneventful. Minor membrane exposures were observed. Defects with membrane exposure displayed an inflammatory infiltrate underneath the membrane. Bone regeneration of trabecular nature, apparent in all animals, was generally limited to the very apical aspect of the defects. Cementum regeneration was limited without obvious differences between experimental conditions. Comparing rhTGF‐β to control defects, statistically significant differences were found for area (1.8±0.4 and 1.3±0.6 mm 2 , respectively: p<0.05) and density (0.3±0.1 and 0.2±0.03. respectively: p<0.05) of alveolar bone regeneration. Observed differences are small and represent a clinically insignificant potential for enhanced regeneration in this preclinieal model. Within the limitations of study, it may be concluded that rhTGF‐β 1 has a restricted potential to enhance alveolar bone regeneration in conjunction with GTR.