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Hyper‐reactive peripheral neutrophils in adult periodontitis: generation of chemiluminescence and intracellular hydrogen peroxide after in vitro priming and FcγR‐stimulation
Author(s) -
Fredriksson M.,
Gustafsson A.,
Åsman B.,
Bergström K.
Publication year - 1998
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1998.tb02461.x
Subject(s) - intracellular , priming (agriculture) , periodontitis , lipopolysaccharide , stimulation , hydrogen peroxide , reactive oxygen species , chemistry , chemiluminescence , tumor necrosis factor alpha , immunology , porphyromonas gingivalis , medicine , endocrinology , biology , biochemistry , botany , germination , organic chemistry
. We have earlier reported a higher Fcγ‐receptor (FcγR)‐mediated generation of reactive oxygen species, measured as luminol‐enhanced chemiluminescence (CL) from peripheral neutrophils in adult periodontitis patients. The aims of this study were to confirm our previous results and lo elucidate the mechanism of this phenomenon by measuring CL in parallel with the intracellular production of hydrogen peroxide, after stimulation with opsonized bacteria. To determine whether the higher CL was associated with altered responsiveness to priming, the cells were preincubated with tumor necrosis factor α (TNFα) and lipopolysaccharide (LPS). While CL was significantly higher in subjects with periodontitis, there was no difference in hydrogen peroxide production between the patients and the controls, indicating that the hyperreactivity is related to the generation of other oxygen species than H 2 O 2 and/or to processes in the outer cell membrane. The responsiveness to priming with LPS on CL was slightly but not significantly higher in the periodontitis group, suggesting that priming could be of value for distinguishing subjects with periodontitis. When assaying intracellular production of H 2 O 2 , TNFα and LPS had both a priming and an activating effect. There were no significant differences between the two groups. In conclusion, this study shows a higher FcγR‐mediated CL of peripheral neutrophils from adult patients with periodontitis, thus confirming our earlier results. The hyperreactivity seems to be related to the outer cell membrane or to oxygen species other than H 2 O 2 .