Premium
Assessment of in vitro interleukin‐2‐producing capacity of peripheral blood lymphocytes from patients with periodontitis.
Author(s) -
Tkahashi Keiso,
Akutsu Isao,
Arai Hideo,
Sato Nobuhiko,
Nagai Atsushi,
Kurihara Hidemi,
Murayma Yoji
Publication year - 1997
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1997.tb01183.x
Subject(s) - peripheral blood mononuclear cell , periodontitis , ionomycin , medicine , immunology , cd3 , interleukin 2 , lymphocyte , interleukin , monoclonal antibody , cytokine , stimulation , in vitro , antibody , endocrinology , biology , immune system , cd8 , biochemistry
In this cross‐sectional study, we assessed the in vitro interleukin‐2 (IL‐2) producing capacity of peripheral blood mononuclear cells (PBMC) and lymphocytes from patients with different forms of periodontitis. 45 patients (12 with localised early onset periodontitis (LEOP), 20 with generalised early onset periodontitis (GEOP), and 13 with adult periodontitis (AP), and 20 periodontally healthy subjects (HS), participated in this study. PBMC and lymphocytes were isolated from the subjects and their cells were stimulated with an anti‐CD3 monoclonal antibody (anti‐CD3 MoAb) and the secreted IL‐2 levels in the culture were bioassayed. No significant differences could be found in IL‐2 producing activity of PBMC between the patients and HS group. There was wide interindividual variation and high and low “IL‐2 producers” were noted. We found a LEOP patient who was a high producer of IL‐2 (>mean+8 SD) and 2 LEOP patients and a HS who were low producers of IL‐2 (