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The effect of interleukin‐1 (IL‐1) on androgen metabolism in human gingival tissue (HGT) and periodontal ligament (PDL)
Author(s) -
Kasasa S.C.,
Soory M.
Publication year - 1996
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1996.tb00568.x
Subject(s) - androstenedione , medicine , endocrinology , periodontal fiber , dihydrotestosterone , testosterone (patch) , androgen , chemistry , metabolism , nandrolone , connective tissue , anabolism , biology , hormone , dentistry , pathology
Due to the potent anabolic effects of the androgenic metabolite 5α‐dihydrotestosterone (DHT) on matrix synthesis by connective tissue and bone, it was pertinent to investigate the effects of interleukin‐1 (IL‐1) on androgen metabolism by chronically inflamed human gingival tissue (HGT) and periodontal ligament (PDL). Duplicate incubations of HGT and PDL derived from 6 subjects (age‐ and sex‐matched) were performed in Eagle's MEM+FCS with 14C‐testosterone to study baseline conversion to DHT and 4‐androstenedione. Similarly further incubations were performed for 24 h in a 5% CO 2 in air incubator, with HGT and PDL from 4 comparable patients to study the effect of IL‐1 on this conversion. The medium was extracted radioactive metabolites separated by thin‐layer chromatography and quantified. When baseline metabolism of HGT was compared with that of PDL. both tissues metabolised 14C‐testosterone to DHT and 4‐androstenedione. There was a 2.4‐fold increase in DHT synthesis by PDL over that of HGT ( n =6; p <0.005) and a 2.5‐fold increase in 4‐androstene‐dione formation by PDL compared with HGT ( n =6; p <0.01). In response to IL‐1, HGT demonstrated a 2‐fold increase in DHT synthesis ( n =4; p <0.005) and a 3.5‐fold increase in 4‐androstenedione formation ( p <0.01) over control gingival tissue; PDL showed a 9‐fold increase in DHT synthesis in response to IL‐1 ( n =4; p <0.005) and a 6‐fold increase in 4‐androstenedione formation (p<0.005) over control ligament tissue. The increased androgen metabolic capacity of PDL over HGT, both at baseline and in response to IL‐1 is in keeping with protein studies and may be relevant to repair processes during inflammatory periodontal disease.